A Review Of cytometers
A Review Of cytometers
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Information-enriched characterization of mobile populations. Morphological details from photographs can incorporate into the richness of flow cytometry facts. As an example, the determine displays an in any other case conventional apoptosis assay using Annexin V and PI, including cell imaging to characterize cells in Each individual population to reveal morphologically unique functions. These insights couldn't have been acquired from multiplex staining alone.
Whether or not you’re a rookie or a specialist, explore our sources to advance your exploration and skills in flow cytometry.
Blend acoustic focusing technology with significant-parameter Assessment for versatile spectral unmixing and conventional workflows.
Explore new insights into your experimental facts by visualizing populations working with blended fluorescence and morphology details. Our application interface wizards allow downsampling, concatenation (merged files) and dimensionality instruments that happen to be created to the program for an intuitive, streamlined info Examination working experience.
"An image is just not very well worth 1000 dots, however it genuinely really helps to see photographs. It is great to check out illustrations or photos that correlate with staining. By way of example, AnnexinV-good cells which are morphologically different than healthful cells, or CD14+ monocytes that are bigger and a lot more textured than CD3+/CD4+/CD14- lymphocytes.
On top of that, it's illuminating to discover the quantity of doublets slip by means of regular singlet gates, and fantastic to validate the status of unexpected double-beneficial events in my Evaluation."
Pair your Attune flow cytometer with an autosampler for elevated workflow efficiency, and include robotic integration for max throughput.
Consistent graphic good quality even at large throughput Acoustic concentrating and also a substantial-speed camera Merge to image these T cells persistently at small or significant flow fees. Simply adjust emphasis and digital camera options to satisfy experimental specifications.
The Attune NxT cytometer may be the trustworthy workhorse instrument your lab can trust in for dependable and trustworthy efficiency. The Attune CytPix design features a higher-velocity brightfield camera and AI-pushed graphic Evaluation software, with latest upgrades such as equipment Studying resources and an intuitive user interface, enabling Increased knowledge precision, precision, and quicker insights into sample biology.
Users can check out function visuals in authentic-time to substantiate sample quality, optimize protocols, and document the morphology of each and every cell population recognized for Evaluation. Imaging seize also Rewards from acoustic concentrating, which really helps to place the cells to ensure that a sharp, centered image is attained.
Audio waves permit specific mobile alignment, resulting in Outstanding data good quality and ~10X quicker sample flow premiums.
We Beforehand demonstrated the strength of imaging Motor vehicle-T/Ramos cell interactions. Let’s examine just the inhabitants of greatest desire, the double good gatherings, to learn more. We are able to now use prolonged morphological parameters (right here, circularity and skewness of depth) to further examine the capabilities of these populations and refine gating on these situations, increasing knowledge robustness.
Leverage the strength of Highly developed technology and innovation to accelerate your discoveries. Believe in from the Attune Flow Cytometer loved ones to provide the dependability, pace, and precision that set your function aside.
Flexible payment prepare from Agilent Economic Options assist you to get the important instrumentation you may need for rapid job, or to improve your lab.
At the guts of every Attune Flow Cytometer lies our revolutionary acoustic focusing technologies, which utilizes audio waves to meticulously align cells throughout the flow cell. This revolutionary solution surpasses common hydrodynamic solutions, leading to a lot quicker processing speeds, Excellent info high quality, and increased sample throughput.
The Attune Xenith cytometer is often a high-parameter website spectral analyzer, offering each spectral unmixing and standard flow capabilities for detailed and flexible cell Investigation.
Agilent Benefit Assure guarantees you at least 10 years of instrument use in the date you buy. Otherwise, We're going to credit rating you with the residual value of the process towards an upgraded model.
Right here, we show that by gating tactics working with graphic-based mostly quantitative parameters can distinguish interacting cells from coincident functions to extra accurately examine interacting cells.